There are various tools for predicting the physical properties using the sequence information. Some of the major ones are discussed below:  prediksi hk 2d jitu malam ini

1. Compute pI/Mw.

2. ProtParam.

3. SAPS.

4. Predicting protein hydrophobicity.

5. PEST and PESTfind.

6. Coils, Paircoil and Multicoil.

1. Compute pI/Mw:

This tool calculates the isoelectric point and molecular weight of an input sequence.

2. ProtParam:

It is a tool which allows the computation of various physical and chemical parameters for a given protein.

3. Predicting protein hydrophobicity:

ProtScale can be used to predict the hydrophobicity of a protein.

4. PEST and PESTfind:

PEST stands for Proline, Glutamic acid, Serine and Threonine residues.

PEST identifies possible PEST regions in a submitted probe using molecular fraction of the P, E, S and T components and the hydrophobicity index of the region.

5. Coils, Paracoils and Multicoil:

This program compares the sequence of a database of known parallel two-stranded coiled-coils and derives a similarity score.

Paircoil predicts the location of coiled-coil regions in the sequence.

Mulitcoil program predicts the location of coiled-coil regions in the amino-acid sequences and classifies the predictions as dimeric or trimeric.

Secondary structure analysis and prediction

The important secondary structure prediction methods are:

1. Chou-Fasman method: