There are various tools for predicting the physical properties using the sequence information. Some of the major ones are discussed below: prediksi hk 2d jitu malam ini
1. Compute pI/Mw.
4. Predicting protein hydrophobicity.
5. PEST and PESTfind.
6. Coils, Paircoil and Multicoil.
1. Compute pI/Mw:
This tool calculates the isoelectric point and molecular weight of an input sequence.
It is a tool which allows the computation of various physical and chemical parameters for a given protein.
3. Predicting protein hydrophobicity:
ProtScale can be used to predict the hydrophobicity of a protein.
4. PEST and PESTfind:
PEST stands for Proline, Glutamic acid, Serine and Threonine residues.
PEST identifies possible PEST regions in a submitted probe using molecular fraction of the P, E, S and T components and the hydrophobicity index of the region.
5. Coils, Paracoils and Multicoil:
This program compares the sequence of a database of known parallel two-stranded coiled-coils and derives a similarity score.
Paircoil predicts the location of coiled-coil regions in the sequence.
Mulitcoil program predicts the location of coiled-coil regions in the amino-acid sequences and classifies the predictions as dimeric or trimeric.
Secondary structure analysis and prediction
The important secondary structure prediction methods are:
1. Chou-Fasman method: